Overview

Contents

Each electron microscope (EM) has its own set of suggested parameters for magnification, suggested gold size, image size, etc.  Generally, many of these settings are common throughout all the EM at NCMIR, but sometimes the differences can result in large change to the end product. 

This page houses a collection of notes and tips for the different EM.

JEOL 4000 #1

Magnification

Minimum Gold Size

2000

Preferably 30 nm, although sticking issues have been noted.  Use 20 nm as an alternative if all else fails

25000

10 nm optimal

30000

10 nm optimal

  • 3.5k x 3.5k image area
  • +/-60 degree tilt holder available for double tilt use

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